BODIPY Conjugates for Microscopy and Single-Molecule Tracking | Protocol Preview
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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking - a 2 minute Preview of the Experimental Protocol
Santosh Adhikari, Chiranjib Banerjee, Joe Moscatelli, Elias M. Puchner
University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), School of Physics and Astronomy; Middlebury College,;
Conventional BODIPY conjugates can be used for live-cell single-molecule localization microscopy (SMLM) through exploitation of their transiently forming, red-shifted ground state dimers. We present an optimized SMLM protocol to track and resolve subcellular neutral lipids and fatty acids in living mammalian and yeast cells at the nanoscopic length scale.
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