Escherichia coli detection in Non sterile pharmaceutical products..?! Bioburden Test..
Escherichia coli detection in non sterile pharmaceutical products..??
Analysis of pharmaceutical products..??
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Bio-burden test
Microbial limit test
Microbial enumeration test
Sample preparation (finished product or raw material)
Recovery methods of microorganisms in the presence of product
Tests for specified microorganisms
Bile-tolerant gram-negative bacteria
Escherichia coli
Salmonella Sp.
Pseudomonas aeruginosa
Staphylococcus aureus
Clostridia
Candida albicans
Acceptance criteria for microbiological quality of non-sterile dosage forms.
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Mix TSB well, and incubate at 30 to 35°C for 18 to 24 hours.???
Shake the container; transfer 1 ml of soybean–casein digest broth to 100 ml of macconkey broth. (to maintain the pH of media)
Incubate at 42 to 44°C for 24 to 48 hours.(for selection faecal coliforms)
Subculture on a plate of macConkey agar then incubate at 30 to 35°C for 18 to 72 hours.
Growth of colonies indicates the possible presence of E. coli.
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I will continue explain the bio-burden test in the next videos……. Thanks for joining
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we will discuss the following topics:-
staining techniques in microbiology
sterilization microbiology
types of microbial spoilage
how does disinfectant kill microorganisms
classification of microorganisms
natural disinfectant for disinfectant
natural disinfectant for cleaning
classification of microorganisms
how does disinfectant kill microorganisms
macromolecules biology
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microbiology chapter 4
evaluation of efficiency of sterilization methods
important bacteria in microbiology
unstained preparation of bacteria
enumeration of bacteria from soil
importance of microbial preservation techniques
media preparation in microbiology
growth media in microbiology
staining techniques in microbiology b pharmacy
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spoilage in microbiology
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motility in microbiology
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