Identification & Characterization Of Botulinum Neurotoxin Light Chain Modulators l Protocol Preview
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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators - a 2 minute Preview of the Experimental Protocol
Dejan Caglič, Kristin M. Bompiani, Michelle C. Krutein, Petr Čapek, Tobin J. Dickerson
The Scripps Research Institute, Department of Chemistry; The Scripps Research Institute, Worm Institute of Research and Medicine (WIRM);
The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.
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