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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos - a 2 minute Preview of the Experimental Protocol
Antionette L. Williams, Brenda L. Bohnsack
University of Michigan, Department of Ophthalmology and Visual Sciences, Kellogg Eye Center;
A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.
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