SEC-SAXS data Analysis via EFA deconvolution and Scatter | Protocol Preview
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Analysis of SEC-SAXS data via EFA deconvolution and Scatter - a 2 minute Preview of the Experimental Protocol
Mark D Tully, Nicolas Tarbouriech, Robert P Rambo, Stephanie Hutin
Structural Biology Group, European Synchrotron Radiation Facility Structural Biology Group; Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS,; Diamond Light Source,; Université Grenoble Alpes/CNRS/CEA/INRA/BIG, Laboratoire de Physiologie Cellulaire and Végétale;
SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.
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