FlyBase Tutorial 2026: Complete Guide to Every Tool & Feature
Learn how to use FlyBase in this complete beginner-friendly FlyBase tutorial for Drosophila researchers, students, and scientists.
In Part 1 of the FlyBase Masterclass, I explain the complete FlyBase homepage and introduce its major tools, search functions, databases, and research resources. You will learn what each section does, when to use it, and how FlyBase can support genetics, genomics, molecular biology, neuroscience, and bioinformatics research.
Topics covered in this video include:
• What FlyBase is and why Drosophila researchers use it
• FlyBase homepage structure
• Jump to Gene versus Search FlyBase
• QuickSearch and Data Class searches
• Gene Groups, pathways, and protein domains
• Gene Ontology and phenotype searches
• Expression and GAL4 searches
• Human disease and homolog searches
• FlyBase BLAST
• JBrowse genome browser
• ID Validator
• Batch Download
• Vocabularies and controlled terms
• FlyBase downloads and external resources
• Community, help, and literature tools
• How to choose the correct FlyBase tool for your research question
This video is suitable for undergraduate students, graduate students, PhD researchers, postdoctoral researchers, teachers, and anyone beginning Drosophila research.
This is Part 1 of the FlyBase Masterclass. Future videos will explain Gene Reports, alleles, mutant stocks, GAL4 drivers, expression data, phenotypes, JBrowse, BLAST, sequence downloading, QueryBuilder, homolog searches, and advanced FlyBase workflows.
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