Gene editing the powerhouse of the cell
In addition to the nucleus, the mitochondria within a cell also contain DNA. Mutations in this mtDNA can cause different diseases. Understanding these diseases and mitochondrial biology in general would be greatly aided if there was a tool that could edit the mtDNA. Whilst CRISPR systems are effective for nuclear gene edits, they currently cannot be used for mtDNA edits. In this video i will discuss a base editor system that can be targeted to mtDNA developed in David Liu's lab.
Timestamps;
00:00 - Intro
01:40 - Gene editing & CRISPR
03:31 - Base editing
06:56 - Editing mitochondrial DNA
10:11 - Efficiency & Off-targets
13:50 - Applications
Paper;
Mok, B.Y., de Moraes, M.H., Zeng, J. et al. A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature (2020). https://doi.org/10.1038/s41586-020-24...
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