Microscope Parts arts And Functions// Blood Microscopic .
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microscope -
Introduction
Types of microscope
Parts of microscope
Introduction
• In 1665 Robert Hooke developed a first laboratory
compound microscope.
• In 1672 Leeuwenhoek developed a first simple microscope with a
magnification of 200x-300x.
He is called as
Father of microscopy.
1) light microscope -
Types of microscope -
1). Light microscope
2). Dark field microscope
3). Fluorescence microscope
4). Electron microscope
Partr of microscope
Illuminator - This is the light source located below the specimen.
Condenser - Focuses the ray of light through the specimen.
Stage -The fixed stage is a horizontal platform that holds the specimen. Objective - The lens that is directly above the stage.
Nosepiece - The portion of the body that holds the objectives over the
stage.
diaphragm - Regulates the amount of light into the condenser.
Base - Base supports the microscope which is horseshoe shaped.
Coarse focusing knob - Used to make relatively wide focusing adjustments
to the microscope.
Fine focusing knob - Used to make relatively small adjustments to the microscope.
eyepiece - Lens on the top of the body tube. It has a magnification of 10x normal vision.
Lenses -
Low power lence - 10X
-Concave mirror
Working distance - 4-8 mm
High power lence- 40X
- Concave mirror
Working distance - 0.5- 0.7 mm
OIL IMMERSION - 100X
-Plane mirror
Working distance - 0.1 mm
Magnification
It is the ratio of the size of an object seen under microscope to the actual size
Resolving power
It is the ability to differentiate two close points as separate. The resolving power of human eye is 0.25 mm
The light microscope can separate dots that are 0.25pm . The electron microscope can separate dots that are 0.5nm .
Working distance
It is the distance between the objects
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