Two-photon Laser Microscope for Cell Membrane Repair Assay | Protocol Preview
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Cell Membrane Repair Assay Using a Two-photon Laser Microscope - a 2 minute Preview of the Experimental Protocol
Joshua J. A. Lee, Rika Maruyama, Hidetoshi Sakurai, Toshifumi Yokota
University of Alberta Faculty of Medicine and Dentistry, Department of Medical Genetics; Kyoto University, Center for iPS Cell Research and Application;
Cell membrane wounding via two-photon laser is a widely used method for assessing membrane resealing ability and can be applied to multiple cell types. Here, we describe a protocol for in vitro live-imaging of membrane resealing in dysferlinopathy patient cells following two-photon laser ablation.
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