GoldenPiCS with OpenCloning: Step-by-Step MoClo Cloning Tutorial
Learn how to use OpenCloning to perform efficient and reproducible cloning using GoldenPiCS templates and the MoClo system.
In this tutorial, we walk you through:
Cloning transcriptional units into BB2 and BB3 plasmids
Assembling a final BB3 plasmid using Golden Gate Assembly
Integrating the plasmid into the K. phaffii genome via homologous recombination
🔬 What is OpenCloning?
OpenCloning is an open-source platform designed to make molecular biology experiments reproducible and shareable. It provides standardized templates for cloning workflows and allows users to replicate, modify, and validate experiments in a transparent, collaborative way.
🌐 Reproduce this experiment at: https://opencloning.org
🎯 Ideal for:
Synthetic biologists
Lab instructors and students
Researchers working with K. phaffii or modular cloning systems