ELISA Based Binding & Competition: Rapidly Determine Ligand-Receptor Interactions l Protocol Preview
Watch the Full Video at https://www.jove.com/v/53575/an-elisa....
An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions - a 2 minute Preview of the Experimental Protocol
Mohameedyaseen Syedbasha, Janina Linnik, Deanna Santer, Daire O'Shea, Khaled Barakat, Michael Joyce, Nina Khanna, D. Lorne Tyrrell, Michael Houghton, Adrian Egli
University of Basel, Applied Microbiology Research, Department of Biomedicine; ETH Zurich, and Swiss Institute of Bioinformatics, Department of Biosystems Science and Engineering; Swiss Institute of Bioinformatics,; University of Alberta, Li Ka Shing Institute for Virology; University of Edinburgh, Regional Infectious Diseases Unit; University of Alberta, Faculty of Pharmacy and Pharmaceutical Sciences; University of Basel, Infection Biology, Department of Biomedicine; University Hospital Basel, Clinical Microbiology;
The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: / jovejournalofvisualizedexperiments