We use the MEGA tool to calculate the synonymous (ka) and nonsynonymous (ks) substitution rate and their ratio of duplicated genes in the family. Find the positive selection within the family genes. We also try to find the segmental and tandem duplication between two genes within the family.
How to take CDS sequence of the gene family?
How to alignment the CDS sequence?
How to use MEGA to calculate the Ka Ks values?
How to convert fasta to table file?
How to convert table to fasta file?
How to convert text to column file?
How to convert text to column?
How to alignment the cds sequence?
How to convert CDS to protein?
How to rename the IDs?
How to make the gene IDs similar to GFF file?
How to use tbtool?
How to calculate the paralogs?
How to determined the purifying evolution?
How to calculate the diversifying evolution?
How to calculate the neutral mutation?
How to determined the segmental duplication?
How to dertermine the tendem duplication?
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