HPLC and GC - what is the difference - animation HD
This is a quick video about the difference between HPLC and GC. Want to know more about HPLC? see my uploaded videos.
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The difference between HPLC (High Pressure Liquid Chromatography) and GC (Gas Chromatography) can be described in four main differences.
Learn the basic concept of High Pressure Liquid Chromatography
01. Introduction
• HPLC - High Perfermance Liquid Chromatogra...
02. The Mobile Phase
• HPLC - What is the function of the mobile ...
03. The Stationary Phase
• HPLC - The Stationary Phase - Animated
04. Normal Phase HPLC vs Reverse Phase HPLC
• HPLC - Normal Phase vs Reverse Phase HPLC...
05. HPLC Isocratic vs Gradient analysis
• HPLC - Isocratic vs Gradient Elution - Ani...
06. HPLC - UV-VIS detection of analytes
• HPLC - UV / VIS detection of analytes Anim...
07. HPLC - How to read a chromatogram?
• HPLC - How to read Chromatogram Easy Expla...
08. What is the difference between HPLC and GC?
• HPLC and GC - what is the difference - ani...
1. Mobile phase
HPLC uses liquid as carrier and GC uses gas as carrier.
2. Column
HPLC uses a column with a length between 5 to 25 cm. In GC analysis columns from 10 to 30 meters are used.
3. Separation
In HPLC separation takes places based on the polarity of the compounds. In GC the compounds are often forced to separate with temperature.
4. Compounds
Generally, non-volatile analytes cannot be directly analyzed using GC. Sometimes derivatization will be helpful to turn non-volatile compounds into volatile ones to be analyze using GC, but it does not always work.
HPLC, using liquid as mobile phase, can analyze compounds which can be dissolved in liquid. Volatility is not that important here. Immediately, we see HPLC can be used on much more analytes than GC, because there are a lot of non-volatile compounds can be dissolved (at least a little bit) in water, methanol, acetonitrile, acetone, or hexane, etc. Therefore, currently, HPLC is used more often than GC.