Solving the Implementation Challenges of Continuous Feedback Control in Cell Culture
Speaker: Jane Ring, Research Scientist, Integrated Pharm Solutions Applications Development, EMD Millipore Corporation
Traditionally, industrial scale mammalian cell culture processes have been controlled through monitoring of temperature, pH and DO (dissolved oxygen) concentration using a continuous feedback control system developed to maintain these parameters at specific set points. As our understanding of the factors impacting cell culture processes increases, implementation of its principles of Quality by Design into cell culture performance is likewise expanding. Therefore, the need to develop and implement new sensor technologies that enable feedback control based on the measurement of additional parameters such as viable cell density (VCD), dissolved carbon dioxide or glucose concentrations is apparent. As these new sensor technologies are developed, users are challenged with their implementation in the context of fixed bioreactor designs.
Our platform of pilot- and clinical-scale single-use bioreactors addresses this challenge through its Mobius® SensorReady technology, an external circulation loop that is connected to the bioreactors via sterile connectors. This webinar will demonstrate the flexibility and effectiveness of bulk process measurements, and applicability of this sensor technology to both suspension and adherent cell culture processes.