Understanding peak calling and BigWig Files in ChIP-Seq
Follow the tutorial here https://crazyhottommy.github.io/repro...
In this video, I walk you through the process of generating BigWig files from ChIP-seq data using DeepTools. It's essential to understand how to calculate reads per genomic bin and the normalization methods involved. I encourage you to follow along and try generating your own BigWig files using the commands I demonstrate. I also showed you how to use MACS3 to call peaks.