Fluorescence and Compensation in Flow Cytometry
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Fluorophores accept light at a specific wavelength and re-emit it at a higher wavelength, giving each fluorophore an excitation and emission wavelength. Although there is a peak or maximal emission, the range is often several hundred nanometers. This can cause problems where unwanted light enters several detectors giving false positives. Compensation is required to remove this unwanted fluorescence. Careful choice of fluorophores can reduce the amount of compensation needed.
This webinar will cover:
Basic fluorescence principles
What is compensation and why you need to apply it
Compensation controls
How to avoid compensation in small panels
Common rules to help build more complex panels
This webinar is recommended for novices and researchers with limited experience of flow cytometry.
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