Preparation of Dissociated Mouse Cortical Neuron Cultures
Reference: https://app.jove.com/v/562/preparatio...
The process of creating cultures of dissociated cortical neurons from mice involves several steps. First, the mice are euthanized and their brains are removed. The cortex is then dissected out and placed in a solution containing enzymes that break down the tissue. The tissue is then mechanically dissociated using a pipette, which separates the cells from each other.
The dissociated cells are then plated onto a culture dish that has been coated with a substance that promotes cell adhesion. The cells are then incubated in a nutrient-rich medium that contains growth factors and other supplements that promote cell survival and growth.
Over time, the cells begin to form connections with each other, creating a network of neurons that mimics the structure and function of the cortex in vivo. Researchers can then use these cultures to study a variety of neurological processes, including synaptic transmission, neuronal development, and neurodegenerative diseases.