Photo-Induced Cross-Linking:Unmodified Proteins Applied To Amyloidogenic Peptides l Protocol Preview
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Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides - a 2 minute Preview of the Experimental Protocol
Farid Rahimi, Panchanan Maiti, Gal Bitan
University of California, Los Angeles, Department of Neurology, David Geffen School of Medicine; University of California, Los Angeles, Brain Research Institute, Molecular Biology Institute; University of California, Los Angeles, Department of Neurology;
Photo-induced cross-linking of unmodified proteins (PICUP) allows characterization of oligomer size distribution in metastable protein mixtures. We demonstrate application of PICUP to three representative amyloidogenic peptides the 40- and 42-residue forms of amyloid β-protein, and calcitonin, and a control peptide growth-hormone releasing factor.
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