Multiphoton Microscopy for Live cell imaging of Retinal Explants | Protocol Preview
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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy - a 2 minute Preview of the Experimental Protocol
Manuela Lahne, Ryne A. Gorsuch, Craig M. Nelson, David R. Hyde
University of Notre Dame, Department of Biological Sciences; Mayo Clinic, Department of Neurosurgery;
Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.
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