MOOC Cytogenetics 4/5 : Telomere and Centromere FISH staining
Introduction: 00:00
Materials and Solutions: 03:49
DNA fixation and Pre-staining: 04:22
PNA probes hybridization: 06:37
Washing and slide mounting: 08:59
Microscope acquisition and analysis: 10:31
Questions/Answers: 13:57
The CEA runs a cytogenetic laboratory implemented in the 1970’s, which specializes in the detection of chromosome aberrations, mechanisms and consequences. Application to biodosimetry has provided for more than 40 years tools for radiation dose assessment. Cytogenetics methods are useful in the case of biodosimetry (ionizing radiations), but can also be applied in different scientific fields in order to detect and quantify various genotoxic effects.
We have created a first set of 5 MOOCs to transmit the knowledge of the laboratory, they are listed below:
1/5 The Dicentric Chromosome Assay
2/5 The Cytokinesis Block Micronucleus Assay
3/5 The Premature Chromosome Condensation Assay
4/5 The Telomere and Centromere FISH staining
5/5 The multi FISH staining
Enjoy the videos and feel free to contact us for more details or any questions at [email protected]
The MOOC project was supported by the European Joint Program CONCERT through WP6. This project is receiving funding from the European Union’s Horizon 2020 under grant agreement no 662287.
This publication reflects only the authors’ view. Responsibility for the information and views expressed therein lies entirely with the authors. The European Commission is not responsible for any use that may be made of the information it contains.