Trafficking of rice blast fungal effectors (
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Three-dimensional measurement from the appressorium penetration point to the BIC of the rice blast fungus growing in a rice cell at 29 hours post inoculation. Apoplastic effector Bas4 with a translational fusion to EGFP (green) outlined the invasive hypha. The BIC and the rice nucleus were visualized by cytoplasmic effector Pwl2 with a translational fusion to mCherry:NLS (red). Overlap of green and red signals is shown in yellow. Source image is a series of 1 μm confocal optical sections taken over a depth of 23 μm. At the start of the animation, the penetration point is on the left, and the BIC is on the right. As the animation rotates, a penetration point-to-BIC measurement (gray line) is overlaid on the image. The measurement was performed with the dendrite creation algorithm in the Imaris software and has a length of 33.2 μm. Bar = 5 μm.