Super-low Input Carrier-CAGE to Map Transcription Start Site | Protocol Preview
Watch the Full Video at https://www.jove.com/v/59805/transcri....
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE - a 2 minute Preview of the Experimental Protocol
Nevena Cvetesic, Elena Pahita, Boris Lenhard
Imperial College London, Institute of Clinical Sciences, Faculty of Medicine; MRC London Institute of Medical Sciences,; University of Bergen, Sars International Centre for Marine Molecular Biology;
Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: / jovejournalofvisualizedexperiments