Optimizing Genetic Incorporation-Chemical Probes Into GPCRs l Protocol Preview
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells - a 2 minute Preview of the Experimental Protocol
Robert Serfling, Lisa Seidel, Thore Böttke, Irene Coin
University of Leipzig, Institute of Biochemistry, Faculty of Life Sciences;
A facile fluorescence assay is presented to evaluate the efficiency of amino-acyl-tRNA-synthetase/tRNA pairs incorporating non-canonical amino-acids (ncAAs) into proteins expressed in mammalian cells. The application of ncAAs to study G-protein coupled receptors (GPCRs) is described, including photo-crosslinking mapping of binding sites and bioorthogonal GPCR labeling on live cells.
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