How To Create Real-Time PCR Primers Using Primer-BLAST
This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.
🎓 MASTERING QPCR ONLINE COURSE 🎓
https://courses.toptipbio.com/p/maste...
In this video tutorial, I will show you how to create real-time PCR primers by using NCBI's Primer-BLAST free online software.
The Primer-BLAST software is great since it uses the Primer3 algorithm to design optimal primers, while simultaneously blasting these sequences on the genome. This ensures the primers will not amplify off-target regions.
THE ONLINE GUIDE
http://toptipbio.com/real-time-pcr-pr...
NCBI WEBSITE
https://www.ncbi.nlm.nih.gov/
VIDEO BREAKDOWN
Step 1: Finding your gene variant (00:18)
Step 2: Loading Primer-BLAST (01:53)
Step 3: The Primer-BLAST settings (02:22)
Step 4: The primer reports (08:40)
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