Determine Ice-Binding Planes-Antifreeze Proteins: Fluorescence-Based l Protocol Preview
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Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity - a 2 minute Preview of the Experimental Protocol
Koli Basu, Christopher P. Garnham, Yoshiyuki Nishimiya, Sakae Tsuda, Ido Braslavsky, Peter Davies
Queen's University, Department of Biomedical and Molecular Sciences; Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke; National Institute of Advanced Industrial Science and Technology, Research Institute of Genome-Based Biofactory; The Hebrew University of Jerusalem, The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition;
Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.
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