Analyze Evolutionary Relationships Between Two Genomes using NCBI's Comparative Genome Viewer (CGV)
This is a tutorial showing how to use CGV to view assembly-assembly alignments, including cross-species alignments. The Comparative Genome Viewer (CGV) became available in 2022 and continues to be updated and expanded.
https://www.ncbi.nlm.nih.gov/genome/cgv
Use the Feedback form on the CGV home page to request new alignments.