Ep15 size exclusion / gel permeation chromatography cont. - UC San Diego - NANO 134 Darren Lipomi
Continuation of our discussion of size-exclusion or gel permeation chromatography. Concept of void volume, retention volume, internal volume, and distribution coefficient. Calibration curve, stiff vs. flexible polymer chains, and detectors.
Error: at 22:25, I should have written "same degree of polymerization" not "same M". For the same degree of polymerization, polystyrene will have a much larger radius of gyration than polyethylene.