Mass Spectrometry-based Identification of RNA-binding Regions | Protocol Preview
Watch the Full Video at https://www.jove.com/v/56004/sample-p....
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions - a 2 minute Preview of the Experimental Protocol
Robert Warneford-Thomson, Chongsheng He, Simone Sidoli, Benjamin A. Garcia, Roberto Bonasio
University of Pennsylvania Perelman School of Medicine, Epigenetics Institute; University of Pennsylvania Perelman School of Medicine, Department of Cell and Developmental Biology; University of Pennsylvania Perelman School of Medicine, Department of Biochemistry and Biophysics; University of Pennsylvania Perelman School of Medicine, Graduate Group in Biochemistry and Biophysics;
We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: / jovejournalofvisualizedexperiments