Biological Structures With Fluorescence Photoactivation Localization Microscopy l Protocol Preview
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy - a 2 minute Preview of the Experimental Protocol
Nikki M. Curthoys, Michael J. Mlodzianoski, Dahan Kim, Samuel T. Hess
University of Maine, Department of Physics and Astronomy;
We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single cells.
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