This was the first run of All Bosses to kill mech bosses on night 3. Lag at the end was due to PC overheating - I was streaming and it was 35'C in the room. I've since got a new PC.
My new WR of 2'11'30 as of August 14: • All Bosses in 2:11:30 NMA Random Seed Spee...
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My lack of practice in late game caught up to me, costing a bit of time when I burned all my money on reforges instead of saving for moon lord -- I could've tanked the whole fight with nurse, and for that lack of money I desperately sold the fragments, so I couldn't craft lunar wings after Moon Lord.
The earliest ruby that I could see looking back was at 48 minutes -- lots of previous runs blend in together, and I had no idea that I was missing a ruby for King Slime, so I never got one.
First cave & Houses - 0:00
Jungle - 3:32
Stained glass & Hellevator - 8:18
Vertebrae farming - 16:00
Underworld - 21:24
Altars & Crimson farming - 26:18
Hardmode ores - 31:22
Destroyer - 35:42
Skeletron, 30 bones - 41:26
Minor farming - 44:58
Skeletron Prime, Twins - 59:25
Sleeping before Plantera - 1:06:56
Truffle worm - 1:16:26
Hardmode Jungle - 1:21:25
Plantera - 1:33:40
Temple & Golem - 1:35:08
Cultist - 1:40:50
Stardust, Vortex - 1:45:13
Nebula Pillar - 1:55:00
Solar Pillar - 2:00:47
Moon Lord - 2:08:06
Hardmode bosses - 2:11:43
Queen bee & King Slime - 2:27:40