Forward Pipetting Technique with a Manual Micropipette
This video demonstrates the proper technique for forward pipetting. This video demonstrates how a laboratory professional transfers 500 microliters of liquid from one buffer to another (buffer A to buffer B) using a 100 to 1000 microliter micropipette. The process includes setting up a clean workspace, using the proper personal protective equipment, selecting the correct size micropipette, adjusting the volume, mounting the pipette tip, pre-wetting, aspirating, transferring, dispensing the liquid, and finally discarding the pipette tip.