Altogen Labs Apoptosis Assay Service
Altogen Labs http://altogenlabs.com provides apoptosis assay services http://altogenlabs.com/pre-clinical-r.... Cysteine-Aspartic Proteases (Caspases) are a group of proteases that have essential roles in programmed cell death.
Colorimetric/fluorometric/luminescent detection of caspase activity is a standard detection method for apoptosis quantification in tissue-specific models. Detection of caspase activity is achieved through the addition of substrate that, once cleaved by the protease, yields a fluorescent/colorimetric/luminescent signal.
-Signaling cascade leads to activation of Caspase 3/7 which ultimately leads to programmed cell death (Apoptosis)
-Caspase 3 cleavage and activation by Caspases 8, 9, and 10 leads to cleavage and activation of Caspases 6 and 7 by Caspase 3.
Caspase 7 is cleaved and activated by Caspase 3, 9, and 10.-Nucleophilic attack by an active site Cysteine initiates peptide bond cleavage
-Caspase 3/7 Assay for Apoptosis
Uses Caspase 3/7 substrates in a buffer system optimized for caspase activity, luciferase activity, and cell lysis
Log phase cells are transferred to 96-well plates in 200μL of media
Cells are incubated with a compound of interest at a single concentration
Cells are harvested at different time points post-treatment
Caspase reagent is added to the wells resulting in lysis followed by substrate cleavage and generation of fluorescent product
-Caspase 3/7 Assay for Apoptosis
-Mammalian Cell
Induction of Apoptosis activates Caspases 3 and 7
After cell lysis, the Caspase reagent is cleaved producing a fluorescent signal that can be measured and quantified
-Altogen Labs has a professional staff experienced with Apoptosis assays
Altogen Labs is compliant with the FDA’s Good Laboratory Practice guidelines
Altogen Labs has a vast array of cell lines at its disposal for Caspase 3/7 Apoptosis assays including HepG2 (Hepatocellular Carcinoma) and MCF-7 (Breast Adenocarcinoma)
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