#Bioedit
In this video, we will make the arrangement of our gene sequence that we get after cloning from sequencing result. We merged the farward primer sequencing and reverse primer sequencing results of our gene, edit or remove the primer or ambiguous bases and make it 100% pure sequence. Then We will make the alignment with the sequence that we have downloaded from #Phytozome or any other data base to check wither our cloned gene’s sequence is similar to that sequence or have some mutation. After that we can use the sequence in further over expression or knockdown or other experiments used for characterization of the gene or use to prove the function of the gene.
We show the clustalw alignment. Used the Genome.jp for alignemnt
BioEdit is one of the most common programs used in molecular biology studies. It was developed initially as a biological sequence alignment editor written for Windows only. It contains many features for sequence alignments modes of easy hand alignment, Split window view, user-defined color, information-based shading, and auto integration with other programs such as ClustalW and Blast. However, in the last few years, it was developed dramatically to integrate many other features and functions and use molecular tools for molecular biologists such as several modes of hand alignment, plasmid drawing and annotation, restriction mapping, and much more. It became one of the widely used programs in molecular biology with its multipurpose tools in molecular biology. The freeware license and its efficient up to date modules besides its quick ability to produce results make it one of the most popular programs for molecular biologists nowadays.
#SequenceAnalysis
#ConsensusSequences
#BioEditSequenceAlignmentEditor
#FarwardReversePrimer