Two-Photon Microscope for in Gastrula for Controlled Volume Ablations | Protocol Preview
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Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula - a 2 minute Preview of the Experimental Protocol
Arthur Boutillon, Sophie Escot, Nicolas B. David
CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris, Laboratory for Optics and Biosciences;
Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment.
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