Integrated System: Upconversion Nanoparticle-Mediated Kinase Photoactivation l Protocol Preview
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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation - a 2 minute Preview of the Experimental Protocol
Hua-De Gao, Pounraj Thanasekaran, Tzu-Ho Chen, Yu-Hsu Chang, Yu-Ju Chen, Hsien-Ming Lee
Academia Sinica, Institute of Chemistry; National Taiwan University, Department of Chemistry; National Taipei University of Technology, Department of Materials and Mineral Resources Engineering;
In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.
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