Will highly acidic or basic samples affect an HPLC C18 column?
Should you worry about the pH of a sample damaging the column? What happens when your sample isn’t in the same pH as the mobile phase? I break it all down in simple terms!
So this is in response to a video I did about the use of guard columns. https://axionlabs.com/chromatography-...
The general rule in HPLC is that we always want to dissolve the sample in the mobile phase—that is the ideal situation. If the sample solvent is the same as the mobile phase, it will just disappear; it will blend in.
In normal cases, pH of your sample won’t damage your column.
Read the blog here: https://axionlabs.com/chromatography-...
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