Phosphoglycerate Kinase (hPGK1) and it's purification
Here's a presentation that I did for a lab meeting, maybe it will help some of you
References Used:
Serimbetov, Z., Baxter, N. J., Cliff, M. J., & Waltho, J. P. (2017). 1H, 15N, 13C backbone resonance assignments of human phosphoglycerate kinase in a transition state analogue complex with ADP, 3-phosphoglycerate and magnesium trifluoride. Biomolecular NMR assignments, 11(2), 251–256. https://doi.org/10.1007/s12104-017-97...
Dhar, A., Samiotakis, A., Ebbinghaus, S., Nienhaus, L., Homouz, D., Gruebele, M., & Cheung, M. S. (2010). Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding. Proceedings of the National Academy of Sciences of the United States of America, 107(41), 17586–17591. https://doi.org/10.1073/pnas.1006760107
Blake C. (1997). Phosphotransfer hinges in PGK. Nature, 385(6613), 204–205. https://doi.org/10.1038/385204a0
Burgess R. R. (2009). Protein precipitation techniques. Methods in enzymology, 463, 331–342. https://doi.org/10.1016/S0076-6879(09...
(wiki page is also very useful): https://en.wikipedia.org/wiki/Phospho...