Types of Light Microscope
Here are the 6 types of light microscopes. So, let's dive into the video.
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NUMBER 1.
Dark-field Microscope
It was invented by Zsigmondy. In a darkfield microscope, a special condenser lens is used with an opaque disc at the center, so that direct rays don’t enter the objective lens. Only light scattered by the specimen enters the objective lens to form a bright image against a dark background. The Darkfield microscope does not have a good resolution. It is used in microbiology and in autoradiography.
NUMBER 2.
Phase-Contrast microscope:
It is a modified light microscope developed by Frederick Zernicke. In this microscope, an annular diaphragm is issued below the condenser lens and a transparency phase plate above the objective. This arrangement in the microscope causes differences in light intensity while passing through the specimen so that denser parts alter the path of light more than the less dense cytoplasmic region. The image formed, therefore, has a varying contrast for different regions.
NUMBER 3.
Ultraviolet Microscope:
Casperson invented the ultraviolet microscope where the illuminating source is ultraviolet rays from mercury or iodine-Quartz lamp. Image is captured on photographic film.
NUMBER 4.
Fluorescence microscope:
It is a modification of an ultraviolet microscope. Fluorescent dyes absorb UV light and emit longer-wavelength light, which is used to stain specific macromolecules of cells. The fluorescence microscope makes the use of two filters, one filter permits only short-wavelength light to pass through the specimen for observation. Another one is used to block light emitted by fluorescent dye. It is used to study photochemical reactions within living cells.
NUMBER 5.
Confocal microscope:
In this case, a laser beam is focused to scan the: specimen, which produces a clear image of one plane of the specimen, while other planes of the specimen are excluded and don’t blur the image.
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