HPLC Pressure Fluctuations Explained: Fix Poor Mobile Phase Degassing Fast
HPLC pressure fluctuations, pressure ripple, baseline noise, and retention time drift are most commonly caused by poor mobile phase degassing and air entering the pump. In this video, we explain why inadequate degassing leads to pressure instability, how dissolved gases form bubbles inside the HPLC pump, and how to fix pressure fluctuations quickly and correctly.
You will learn how to identify degassing-related pressure problems, what causes air ingress in HPLC systems, and the correct priming and purging steps to restore stable pressure. This video also covers preventive best practices, including mobile phase planning, solvent degassing methods, inlet frit maintenance, and reservoir venting.
This practical troubleshooting guide is intended for HPLC users experiencing pressure oscillation, unstable baselines, or poor retention time reproducibility due to degassing or pump inlet issues.
Topics covered:
HPLC pressure fluctuations and pressure ripple
Poor mobile phase degassing in HPLC
Air in the HPLC pump and cavitation
Baseline noise and retention time drift
HPLC pump priming and purging
Preventing degassing-related pressure instability
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00:00 Why HPLC pressure fluctuates
00:20 What pressure ripple and baseline noise mean
00:50 How poor degassing causes pressure instability
01:20 Common causes of air entering the HPLC pump
01:55 How to fix pressure fluctuations step by step
02:35 How to prevent degassing-related pressure problems
02:55 Final summary and key takeaways