assume cs: code ,ds: data
code segment
start:
mov ax,data
mov ds,ax
mov ax,00h
mov al,num1
mov bl,num2
sub al,bl
mov result,al
mov ah,4ch
int 21h
code ends
data segment
num1 db 4Ah
num2 db 3Fh
result db 00h
data ends
end start