Measuring TCR-pMHC Binding In Situ Using FRET-Based Microscopy Assay l Protocol Preview
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Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay - a 2 minute Preview of the Experimental Protocol
Markus Axmann, Gerhard J. Schütz, Johannes B. Huppa
Vienna University of Technology, Institute for Applied Physics - Biophysics; Medical University of Vienna, Institute for Hygiene and Applied Immunology;
This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.
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