To honor the 20th anniversary of this logo, the sound has been remastered and remixed by using audacity with editing tools like Graphic EQ and Bass and Treble. It also has been sampled to 384000 Hz in a 32-bit audio for higher quality.
The logo first debuted somewhere between June 16 and 21, 2004, on Nelvana's website. The first television show to use it aside from any older shows and home video releases at the time is Miss Spider's Sunny Patch Friends (I believe...), which debuted on September 7, 2004. The first movie (aside from older movies) to use it was Care Bears: Journey to Joke-a-lot, which was released on October 5, 2004. That year, shows like 6teen, Delta Slate, The Backyardigans, and Pandalian also debuted on television in any country.
In 2006, the widescreen version of the logo was created due to widescreen 720p or 1080p becoming a norm. The long widescreen version of the logo was mostly used on Nelvana DVDs, but the 720p HD version (upscaled to 4K for this video) of it was used in the 2009 Bakugan Battle Brawlers video game. It was sourced from Xbox 360. Special thanks to @ClosingLogosHD for the logo source!