Pittcon 2012 - Measurement Tools for Reactive Oxygen and Nitrogen Species - Abstract 1
This session was webcast at Pittcon 2012 in Orlando, Florida on Tuesday, March 13, 2012.
TITLE: Investigating Oxidative Stress at the Single Cell Level
SPEAKER: Christian A Amatore
ABSTRACT OVERVIEW: Living cells exchange information conditioning their collective behavior in tissue or living organisms through the emission of several chemical messengers including hormones, neurotransmitters as well as small molecules produced by their oxidative stress cellular machineries. The importance of these oxidative stress messengers has been widely recognized by biologists.
We will illustrate the application of the artificial synapse method using ultramicroelectrodes [1,2] to the specific case of bone maintenance. For this purpose, The oxidative stress responses of single MG63 osteosarcoma (bone cancer cells) submitted to a brief mechanical stress have been investigated by amperometry at platinized carbon fiber electrodes for monitoring and characterizing the nature and the amounts of the various reactive oxygen (ROS) and reactive nitrogen species (RNS) released. It was thus shown that, in average, single MG63 cell released prominent amounts of reactive nitrogen species (17 fmol NO, 6 fmol ONOO-, and 5 fmol NO2-) together with a comparatively small quantity of H2O2 (2 fmol). These species resulted from the primary production of 13 fmol for O2- and 28 fmol for NO per single cell as reconstructed from the stoichiometries of the ROS and RNS releases. The high NO/H2O2 and NO/O2- ratios thus found are perfectly consistent with previous claims that the malignant bone formation ability of the osteosarcoma cells is related to a specific high production of NO• associated to a small one of O2-.[3]
Acknowledgements
This work was supported by CNRS, ENS and Xiamen University.
References
1. C. Amatore, S. Arbault, M. Guille, F. Lemaître, Chem. Rev. 108, 2585 (2008).
2. C. Amatore, S. Arbault, A. Koh, Anal. Chem. 82, 1411 (2010).
3. R. Hu R, M. Guille, S. Arbault, C.J. Lin, C. Amatore, Phys. Chem. Chem. Phys. 12, 10048 (2010).