Karyotype, Chromosome Banding, Giemsa and Reverse Giemsa Staining
Chromosome Banding, Giemsa, and Reverse Giemsa Staining, Karyotype lecture - In this video, the details of the karyotype and Chromosomal banding pattern with different staining (Giemsa and Reverse Giemsa) have been described. Also from this tutorial, you will understand why Euchromatin is lighter than Heterochromatin. The basic difference in the pattern of Giemsa and reverse Giemsa technique is also detailed.
The components of Giemsa are 1)methylene blue 2)Azure B, and 3)Eosine. During this staining process, first, mild trypsin is applied which actually partially degrade the protein part of chromatin. As Euchromatin is loosely packed, so, the protein part of euchromatin is easily accessed and partially degraded but heterochromatin is tightly packed and hence protein cannot be degraded due to inaccessibility of trypsin to that part. Therefore, for a less degraded protein of heterochromatin, DNA is also totally packed and retain dark staining by Giemsa. Whereas, due to more degraded proteins of euchromatin, DNA become loosely packed and retain less Giemsa stain.
'Biswa's Coaching' provides basic and advanced level education of Modern and Applied Biological Sciences like Biotechnology, Bioinformatics, Genomics, Proteomics, Molecular Biology and many more related subjects for undergraduate and post-graduate students. Also, this channel covers Computer Programming Languages and Biostatistics. The purpose is to provide right and in-depth information about a topic. I hope you enjoy my video tutorial.
If you have any concern then comment me down or can send email to my email id: [email protected]
You can also reach me on:
Researchgate: https://www.researchgate.net/profile/...
Linkedin:
/ biswanath-chowdhury-a2195026
Tutorindia:
https://tutorindia.net/Tutor_Profiles...
Please subscribe to my channel. Thanks for watching.