TruSeq | TruSeq DNA library Preparation Kits | Illumina Library Preparation |
The Kits are used to prepare DNA
libraries with insert sizes from 300–500 bp for single, paired-end,
and indexed sequencing.
The protocol supports shearing by either
sonication or nebulization with an input requirement of 1 µg of
DNA.
Library construction begins with fragmented gDNA
Blunt-end DNA fragments are generated using a combination
of fill-in reactions and exonuclease activity
An ‘A’-
base is then added to the blunt ends of each strand, preparing
them for ligation to the sequencing adapters (Figures 2C).
Each adapter contains a ‘T’-base overhang on the 3’-end, providing a
complementary overhang for ligating the adapter to the A-tailed
fragmented DNA.
These adapters contain the full complement of
sequencing primer hybridization sites for single, paired-end, and
indexed reads.