Measuring Proteins [ELISA, IQELISA, SIMOA}
White Paper: https://www.raybiotech.com/elisa-immu...
Technology Platforms:
ELISA: https://www.raybiotech.com/elisa-kits/
Immuno-PCR: https://www.raybiotech.com/elisa-kits...
SIMOA: https://www.raybiotech.com/cro-servic...
Speaker: Brittany: / bmpope
The basic design principle of ELISA depends on the stability and specificity of antibody-antigen interactions. Briefly, a capture antibody that is immobilized to a solid substrate, such as a bead or microplate, binds to a protein-of-interest in a variety of sample types. Such samples may include plasma, serum, cell and tissue lysates, conditioned media, cerebrospinal fluid, or urine. When ELISA was first introduced in 1971, an enzyme-labeled antigen was captured by an antibody on cellulose particles.
The antibody-antigen conjugate was then isolated through repeated centrifugation and washing. Since then, different ELISA formats have been developed, including the sandwich ELISA where a target protein is “sandwiched” between a capture antibody and a labeled detection antibody. This ELISA format is highly specific since two antibodies are required to bind to a protein for detection. Moreover, protein concentrations (i.e., quantitative data) can be determined with a standard curve. The ELISA platforms discussed here – standard ELISA, immuno-PCR, and single molecule array (SIMOA) – are sandwich ELISAs that generate quantitative data. However, they differ in terms of their substrates, detection methods, instruments, sample volumes, and sensitivities.
0:00 Sandwich ELISA development
1:05 Standard ELISA
4:06 IQELISA
6:35 SIMOA
9:00 Multiplexing