Cyanobacteria: Conjugation By CyanoGate Modular Cloning Toolkit l Protocol Preview
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Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit - a 2 minute Preview of the Experimental Protocol
Grant A. R. Gale, Alejandra A. Schiavon Osorio, Anton Puzorjov, Baojun Wang, Alistair J. McCormick
University of Edinburgh, Institute of Molecular Plant Sciences, School of Biological Sciences; University of Edinburgh, Centre for Synthetic and Systems Biology; University of Edinburgh, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences;
Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.
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