Multicolor Flow Cytometry & SPICE: Data Visualization and Analysis Guide
Explore the comprehensive guide to multicolor flow cytometry data analysis and visualization using FlowJo and SPICE (Simplified Presentation of Incredibly Complex Evaluations). This video provides step-by-step instructions for preparing and analyzing data, creating combination matrices, and producing professional visualizations like pie charts, bar plots, and more. Whether you're a beginner or an experienced researcher, this tutorial will enhance your skills in handling complex datasets.
🎯 *What You'll Learn:*
Essentials of SPICE and FlowJo software.
How to prepare flow cytometry data for combination analysis.
Using PESTAL software for advanced matrix management.
Creating and customizing visualizations like pie charts, bar plots, and more.
Exporting results into publication-ready formats.
🔗 *Resources Mentioned:*
SPICE Software: https://niaid.github.io/spice/
FlowJo Media YouTube Channel: / @flowjomedia
Publication Presented in Tutorial:
1. Altered IL-7 signaling in CD4+ T cells from patients with visceral leishmaniasis
https://doi.org/10.1371/journal.pntd....
2. Increased amphiregulin expression by CD4+ T cells from individuals with asymptomatic Leishmania donovani infection
http://dx.doi.org/10.1002/cti2.1396
📚 Perfect for immunologists, researchers, and students working with flow cytometry and high-dimensional data visualization.
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Time Code:
00:00 - You Tube Chanel Introduction
00:10 - Introduction
00:46 - SPICE Software Introduction
02:48 - FlowJo Software Information and Other Related Details
04:00 - FlowJo Media You Tube Chanel Information
04:27 - Initiation Of Data Analysis Starting From FlowJo analysis
05:28 - Conconate FCS File
07:40 - Working or Flow Data preparation from Conconated file to make combination data
13:00 - Combination Data file creation in FlowJo software
23:54 - Combination Data file creation in excel for further use in PESTAL software
34:45 - Combination Matrix data management by PESTAL software
37:16 - Data Visualization By SPICE software
38:24 - Pie Figure preparation and modification
42:29 - Bar Figure preparation and modification
43:53 - Nplot Figure preparation and modification
44:19 - CoolPlot Figure preparation and modification
44:48 - Student T Test preparation and modification
46:07 - SPICE data export into PDF format