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Tom Jupille and John W. Dolan, LC Resources, present Session 3 of the "HPLC Troubleshooting and Preventive Maintenance: The Keys to Fixing Problems Quickly" course. This 60-minute online learning seminar covers physical problems associated with the use of HPLC columns. This module is a genuine learning tool. All content from this online seminar is applicable to HPLC analysis and methods independent of vendor hardware.