9- General microbiology| Giemsa stain| Preparation| malaria detection and other parasites
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Giemsa stain, named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.
Easy mnemonic to remember Giemsa stain taking organisms is explained in this video. Trypanosomes Plasmodium Rickettsia Borrelia and Chlamydia are stained by Giemsa stain.
Test for differential staining of cells for detection of parasites by Giemsa stain.
PRINCIPLE:
Smears are fixed using the Giemsa Fixative. Slides are immersed in Giemsa Reagent to differentially stain specific cellular components. The cellular components stain either basophilic (blue) or eosinophilic (orange granules). The color intensity can be varied by adjusting the staining time.